Practical Manual on Plant Molecular Biology and Analytical Techniques
₹ 420
Author(s)
:
Dr. S. Girija
Paperback ISBN
:
978-93-89680-43-0
Publisher
:
AkiNik Publications
Language
:
English
Pages
:
60
Publication Year
:
2020
Binding
:
Paperback
Description
This book explores the principles and methodologies of micropropagation, focusing on the techniques used to propagate plants in vitro. It covers the four stages of micropropagation: initiation, multiplication, rooting, and acclimatization, emphasizing the importance of aseptic conditions for success. Additionally, it discusses both direct and indirect regeneration processes, with a detailed guide on growth media preparation, including MS media, and the use of growth regulators like cytokinins and auxins. Through practical experiments and step-by-step procedures, this book provides valuable insights into the rapid, virus-free, and efficient propagation of plants for commercial and research purposes.
Abstract
This book provides an in-depth exploration of micropropagation, a method of plant reproduction that involves culturing plant tissues in vitro to generate genetically identical plants. The process includes stages such as surface sterilization, multiplication, rooting, and acclimatization, requiring strict aseptic conditions. The book focuses on both direct and indirect regeneration techniques, utilizing growth media like MS medium and growth regulators such as BAP, Zeatin, and IAA. By following laboratory protocols and detailed methodologies, readers can learn to propagate plants efficiently, maintain virus-free stocks, and enhance agricultural productivity through tissue culture techniques.