Short Protocols in Molecular Biology

This book offers comprehensive methods for isolating bacterial and plant genomic DNA, focusing on the use of CTAB for plant DNA extraction and proteinase K digestion for bacterial DNA. It presents a straightforward protocol for cell lysis, removal of contaminants such as polysaccharides and proteins, and DNA precipitation. The methods cater to diverse plant species and bacterial strains, ensuring reproducibility and scalability. The protocols, combined with tips for optimizing yields, are essential tools for researchers in molecular biology, offering efficient techniques for DNA extraction and purification.

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