A Text Book on Plant Tissue Culture in Horticulture: Principles, Technologies, and Commercial Applications

This book presents a comprehensive and contemporary account of plant tissue culture applications in horticulture, integrating fundamental principles with advanced technological innovations. It systematically covers laboratory design, aseptic methodologies, nutrient media optimization, and diverse micropropagation techniques for fruit, vegetable, and ornamental crops. The text further explores modern breeding tools such as somatic embryogenesis, protoplast fusion, genetic engineering, and gene editing. Special emphasis is placed on germplasm conservation, cryopreservation, artificial seed technology, commercialization strategies, and emerging trends, including bioreactor-based production and AI-driven tissue culture systems, making it valuable for researchers, students, and industry professionals.