Quantitative estimation of soil physico-chemical properties following suitable analytical methods is the basic step towards formulating appropriate soil management decisions and precise nutrient recommendation for increasing crop production. Accuracy of soil analysis majorly depends up on the protocols followed during collection, processing, storage, and analysis of the soil samples. This manual entitled “Laboratory manual on fundamentals of soil science” is being compiled to support students, researchers, and lab technicians with laboratory analysis of key soil physical and chemical properties. Written in simple language, the manual describes analytical principles, procedures, and necessary interpretations for effective soil testing and suitable soil management.