The objective is to provide an introduction to experimental design and basic techniques commonly used in life science research laboratories. The practicals have been devised to cover the topics from analytical techniques, biochemistry, cell and molecular biology, microbiology and bioinformatics. Students will gain expertise in carrying out different experiments of various types and learn the use of several high end analytical tools and techniques.While finalizing the contents of this manual the suggestions of the related faculty members have also been consulted. These technologies are becoming a cornerstone in all facets of modern medicine and biotechnology, students will benefit from a working knowledge of their theory and methods. Therefore, this expanded laboratory manual provides step to step guide for carrying out the experiments. Preparation of reagents and procedures are written in a comprehensive manner for better understanding to instructor, lab assistant and students.