Protocols on Extraction of Nucleic Acid from Different Sources and Preparation of Reagents

This book offers comprehensive protocols for the extraction of genomic DNA and RNA from bacteria, tailored for use in molecular biology studies. Covering both Gram-positive and Gram-negative bacteria, the protocols detail step-by-step procedures for efficient nucleic acid isolation, ensuring high-quality samples for PCR, gene expression analysis, and other molecular techniques. Reagents, preparation instructions, and centrifugation methods are provided, with a focus on minimizing contamination. Ideal for microbiologists, researchers, and students, this resource is an invaluable guide for anyone involved in nucleic acid extraction and bacterial genomic studies.